Published Dec 16, 2020 | Updated Dec 16, 2020. F3/F5 Multi-Mode Microplate Readers Fluorescent signals from the same assay plate were measured on the SpectraMax iD5 reader (the SpectraMax iD3 reader is also suitable for these assays, as it has the same onboard fluorescence performance as the SpectraMax iD5 reader), SpectraMax i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. SpectraMax Glo Steady-Luc Reporter Assay Kit. BMG LABTECH. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 005 OD. CloneMatrix. MetaXpress. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. 00. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. SpectraMax Paradigm Multi-Mode Microplate Reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . ( C ) H2A. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. com A. The door opens, revealing the transport lock that fastens the microplate drawer to the internal frame of the instrument. FilterMax F5 Multi-Mode Microplate Reader. 4 Pages. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Approximately 50 µl of these. 5% (v/v) to avoid toxicity. 3. SpectraMax Glo Steady-Luc Reporter Assay Kit. Neurotransmitter Transporter Uptake Assay Kit. Luminescence (glow) Fluorescence polarization. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. FilterMax F3 & F5 Multi-Mode Microplate Readers. The reactions were analyzed in a fluorometer (F5 Multi-Mode Microplate Reader) at 590 nm after a 120-h incubation. An extra plate was done with all the serial dispersions for the MgO-NPs (in. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 40266 Email: drugdiscoverytech@lifetech. Biofilm quantification and scanning electron microscopy (SEM) analysis. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 6% from 2020 to 2027. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. 2 Pages. All experiments were performed in triplicate. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. FilterMax F3 and F5 Multi-Mode Microplate Readers; Absorbance Readers. By category. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. Imaging by immunofluorescence. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. A microplate reader is laboratory machine used for the estimation of biochemical or. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. SP Bel-Art Petri Dish Plate Reader, Bel-Art Products, a part of SP . After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. They provide absorbance, fluorescence, glow luminescence, and other read modes for. 4 Pages. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by. Sunnyvale, Calif. The supernatant was taken and 0. 425 or. VANTAstar™ Flexible Multi-mode Microplate Reader. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 1 Software Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. x. Learn More. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. FilterMax F3 & F5 Microplate Readers. Assay buffer and 100% DMSO were used as vehicle controls. 9. shopping cart 0 items $0. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . 4 Pages. Background light emission was measured using the luminescence setting on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Techniques). Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. Load prepared PicoGreen plate (step C. Add to cart. 0´ 104 1. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 2 Temperature and cooling rate control. Technical specifications Compatible with these Molecular Devices systems Contact Us Phone: +1-800-635-5577 Email: [email protected]. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. Neurotransmitter Transporter Uptake Assay Kit. Open SoftMax® Pro software. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. The absorption was measured in a spectrophotometer ultraviolet–visible (UV–Vis) Filter Max F5 multi-mode microplate reader; a wavelength of 595 nm was taken as the standard value for bacteria. 3. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. 00. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. Perkin Elmer Wallac Victor2 1420 Multilabel. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The values of luminescence are directly proportional to the number of viable cells. Absorbance at 570 nm was recorded on a CLARIOstar multi-mode microplate reader (BMG Labtech, Germany). This document is provided to customers. FilterMax F5 Multi-Mode Microplate Reader. Synergy HTX and Synergy LX. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 09 CAD. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Click to Contact Seller. FilterMax F3 & F5 Microplate Readers. 3 and higher include optimized reader settings, as well as data analysis, for faster results. Perception (Vial Rack Bottom Label 2D Reader) Microscan. After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. The microplate drawer door must be held open manually while. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). HERM LB 500 with NaI detector Radio HPLC Detector. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Jurkat cells were plated in a 12-well dish at a density of 8 x 10 5 cells in 1 mL media. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. GenePix 4300/4400 Microarray Scanner. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. RNA extraction and reverse transcriptase–quantitative PCR. ” e. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. Aspirate 150 µL from wells C5-F5 and discard. 3 Pages. S. 00. Biofilm quantification and scanning electron microscopy (SEM) analysis. The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. This document is provided to customers. Citations: Dual monochromators increase performance and assay flexibility. Synergy™ H1 Hybrid Multi-Mode Microplate Reader, Wavelength Range: 200 nm to 999 nm, BioTek Instruments (CAT#:. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. FilterMax F3. Synergy HTX laboratory equipment pdf manual download. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 2. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . SpectraMax iD5. . Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. MetaMorph NX. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). To do so, perform one of the. Laser-aided direct metal deposition of metals and alloys. Plate Formats:. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. PHA content was calculated based on the equation (fluorescent intensity/OD 600 × 0. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 713579). Each condition was performed in. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. 4. When the user applies a set temperature of 37°C, the top is heated to 37. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. ” e. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. time plots at 30, 60, and 90 min using MATLAB ® R2017b (9. The OD 595 of the culture in the plate (light path length: ∼6. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular devices, CA, USA). #E1910) in a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). The Molecular Devices FilterMax F5 Multi-Mode Plate Reader was tested for compatibility with Life Technologies’ Z'-LYTE® assays using the Z'-LYTE® Tyr6 kit (PV4122) against. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97. Cells were harvested for luciferase assay 48 h after transfection. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. 713579). Transfluor Technology. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. MetaFluor Software. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . SpectraMax Quant dsDNA Assay Kits. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Description: The FilterMax™ F3 features absorbance (visible),. SpectraMax Quant dsDNA Assay Kits. 97059-524EA 84727. CloneMatrix. At day 4 P. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). The ucs load command creates a backup of. 6 Pages. 0. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. ScanLater Western Blot Detection System. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. This microplate reader also features BioTek’s Hybrid Technology, which. 020 fmol 0. , call 1-800-635-5577. Absorbance microplate assays For more than 30 years, absorbance-based detection has been the preferred mode for many microplate-based assays, such as ELISA, protein, and nucleic acid quantitation or enzymatic assays. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. 3 Pages. Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 00 USD. 6. A microplate consists of small wells wherein separate reactions take place. 0´ 104 6. Microplate Readers. 7. Instrument Configuration: Assay Technology Adapta® & The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Turn on the power switch on the back of the instrument. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. 4. Transfluor Technology. Caspase 3/7 activity was performed as previously described [8,9,10,20]. In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular. Upgradeable modules are also available including western blot, cell imaging, and fast. MetaMorph NX. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. Time-resolved Fluorescence. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. The papers, which were published between 2010 and. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. Immunoblot analysis was performed with pooled serum (diluted 1:500) separated by SDS-PAGE under non-reducing conditions and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). 003 fmol 0. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). Safety Information. Time-resolved Fluorescence. Configurability is an important aspect of BioTek’s multi-mode readers to provide the most value for laboratory budgets, but the independent optical systems in our multi-mode readers don’t compromise on performance. 5. 3 Pages. Create an experiment based on the protocol. 9 mM H 2 O 2 for. The experiment was carried out with four biological replicates and two technical replicates. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. SpectraMax Glo Steady-Luc Reporter Assay Kit. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. SpectraMax QuickDrop UV-Vis Spectrophotometer. MDA is one of the final products of LPO. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 1. ** Some compatible filter sets are available for. Delete from my manuals. c. Approximately 50 μl of preculture were inoculated into 1 mL MMAB, leading to a starting density of 0. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). Add to my manuals. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). 1 MB Table of Contents. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. Molecular Devices FilterMax F5 User Manual (49 pages) Multi-Mode Microplate Readers. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. ABS Test results. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. 4 Pages. Topic. 5 μg/ml) in the dark for 1 h. Transfluor Technology. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. #6590-0118) Filter 665/10 (Molecular Devices cat. Thermo Fisher Scientific. 2. The experiment was performed in 4 technical replicates (wells in the cell culture plate). A blank sample was prepared by mixing. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. 9). Homogeneous Time-Resolved Fluorescence (HTRF) . Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. In addition, the instrument features linear and orbital shaking, plus temperature. 4 fmol/100 μl fluorescein in Fluorescence Intensity-Top Read Mode, 0. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Download Table of Contents. Compact designs make these instruments. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). 00. The experiment was carried out with four biological replicates and two technical replicates. Measurement of Caspase 3/7 Activity. ] Example of document to evaluate if the reader . The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. Trinean Dropsense 96 Polychromatic. Molecular Devices Microplate reader VersaMax $1,495. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. $3,000 USD. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. ScanLater Western Blot Detection System. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. 8 Pages. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 666 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. Caspase-3 Activity. The capability of the lixiviated products of the experimental membranes to inhibit the bacterial planktonic growth was estimated from the turbidity of the inoculated culture media at OD λ = 595 nm (filter max F5 multi-mode microplate reader). Plate Formats:. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Coculture experiment. The solution was incubated for 15 min and fluorescence measured on a SpectraMax microplate reader (Molecular Devices) with an. F3/F5 Multi-Mode Microplate Readers. 4) onto the FilterMax F5 to be read with SpectraMax® Paradigm® Multi-Mode Detection Platform. Our newest multimode microplate reader combines simplicity with top-of-the-line performance. SpectraMax M-series Microplate readers. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 020 fmol 0. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Your applications, your modes, your choice. 2 Pages. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 1 (GraphPad Software Inc. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 0´ 104 8. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 8 Pages. 00. Molecular Devices technical support. 00. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. The plate was incubated for 1 hr at room temperature with shaking. Click Run. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). 3 Pages. Statistical analysis was performed using. Samples (180 μl) were stained with 20 μl of Nile red (2. The increase in NADH, an end product in this reaction, was determined by means of its light absorbance at 355 nm using a fluorometric microplate reader (FilterMax F5 Multi-Mode Microplate Reader; Molecular Devices,. 6 reviews. 100 fmol 1536-well (8 µL) 0. Supplier: Molecular Devices. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. 380 or. 4 Pages. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. Activation of TLR4 and TLR2 receptors. 2 Pages. Align the two slots on the bottle holder with the two pegs on the rail. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader.